In Silico Analysis of miR-223 and miR-181b and Their Target Genes in the Pathogenesis of Acute Promyelocytic Leukaemia

Acute promyelocytic leukaemia (APL) is the M3 subtype of acute myeloid (AML). The hallmark for APL diagnosis reciprocal chromosomal translocation between chromosome 15 and 17 to produce PML/RARα fusion gene [1]. combination treatment all-trans retinoic acid arsenic trioxide (ATRA- ATO) 97% effective in treating APL, however molecular mechanism pathogenesis yet be understood. discovery miRNAs epigenetics as potential prognostic biomarkers therapeutic targets various cancers including must further explored. miR-223 downregulation was widely shown associated with worse prognosis previous studies [2]; while miR-181b upregulation studies, although findings varied based on ethnic populations that were involved [3].
 
 In order understand more about roles these miRNAs, this study conducted objective investigate targeted genes regulated by mir-223 mir-181b other cancers, well signaling pathways involving cancers. silico analysis using miRDB 6.0 database identify target filter them relevance significant expression cell lines NB4 HL60. Only a score 80 or higher, 20 RPKM confirmation experimentally validated articles included.
 revealed 162 genes, 612 miR-181b. then obtained comparing predicted articles. 116 found relevant articles, whereas 156 14 level than supported include NXT1, ACSL4, LRRFIP1, NOP2, DENR, AIMP1, DEK, LRRC59, YWHAQ, XRCC5, LAMP2, RAB14, ARF6, ATP6V1B2. On hand, DDX3X, TNPO1, DMXL2, ZFP36L2, PTBP3, SCD, GIGYF1, SLC25A37, PNRC2, TCERG1, RBBP7, WDR82, PRKCD, ACSL4.
 GeneCodis 4 used pathway analysis, which identified 159 KEGG 203 (Table 1 Table 2). There 13 most pertaining Meanwhile, there cancers.
 It can concluded from play important through regulation activation pathways. Nevertheless, functional need investigated experimental validation vitro aid novel APL.